sypro orange protein dye (Lumiprobe)
Structured Review

Sypro Orange Protein Dye, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteorange+protein+gel+stain+5000/ProteOrange+Protein+Gel+Stain%2C+5000%C3%97/pmc12661447-222-6-10
Average 93 stars, based on 11 article reviews
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1) Product Images from "Cyanophycinase is required for heterotrophy in cyanobacteria"
Article Title: Cyanophycinase is required for heterotrophy in cyanobacteria
Journal: The Journal of Biological Chemistry
doi: 10.1016/j.jbc.2025.110791
Figure Legend Snippet: ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.
Techniques Used: Immunodetection, SDS Page, Membrane, Staining, Control, FLAG-tag, Variant Assay
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